Composite
Part:BBa_K3733045:Design
Designed by: Zhenhao Han Group: iGEM21_HZAU-China (2021-10-01)
RNA Thermometer(heat-repressible) test system
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 149
Illegal AgeI site found at 272 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 82
Design Notes
This is a composite part including the RNA thermometer(hot-inhibit) and the neGFP fluorescent protein.
Source
Used a strong RBS to produce high levels of expression of the neGFP fluorescent protein.